amino acids Search Results


bpa uptake assay  (Dojindo Labs)
93
Dojindo Labs bpa uptake assay
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Bpa Uptake Assay, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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advancebio aaa standards  (Agilent technologies)
95
Agilent technologies advancebio aaa standards
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Advancebio Aaa Standards, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amino acid feeding experiment  (Macklin Inc)
86
Macklin Inc amino acid feeding experiment
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Amino Acid Feeding Experiment, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non essential 603 amino acids neaa  (Valiant Co Ltd)
95
Valiant Co Ltd non essential 603 amino acids neaa
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Non Essential 603 Amino Acids Neaa, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nα fmoc nω  (Chem Impex International)
95
Chem Impex International nα fmoc nω
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Nα Fmoc Nω, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5 hydroxydiclofenac  (Toronto Research Chemicals)
92
Toronto Research Chemicals 5 hydroxydiclofenac
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
5 Hydroxydiclofenac, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non essential amino acid solution  (Beijing Solarbio Science)
95
Beijing Solarbio Science non essential amino acid solution
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Non Essential Amino Acid Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast nitrogen base w o amino acids  (Thermo Fisher)
95
Thermo Fisher yeast nitrogen base w o amino acids
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Yeast Nitrogen Base W O Amino Acids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3 bis  (Thermo Fisher)
94
Thermo Fisher 3 bis
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
3 Bis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/3-%5BBis(2-hydroxyethyl)amino%5D-2-hydroxypropanesulfonic+acid%2C+99%25/10__1002_slash_er__7718-44-0-11
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2 amino 4 5 dimethoxybenzoic acid  (Thermo Fisher)
91
Thermo Fisher 2 amino 4 5 dimethoxybenzoic acid
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
2 Amino 4 5 Dimethoxybenzoic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fmoc amino ethoxy acetic acid  (Chem Impex International)
95
Chem Impex International fmoc amino ethoxy acetic acid
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Fmoc Amino Ethoxy Acetic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cystine glutamate antiporter  (Alomone Labs)
92
Alomone Labs cystine glutamate antiporter
AhR activation status does not affect Nrf2 activation or transcriptional activity. RPTECs were cultured under ctrl conditions or subjected to Reox with or without the AhR inhibitor CH223191. (A) Representative western blots of Nrf2 levels (corresponding to its activation status) and the expression of the Nrf2 transcriptional targets xCT <t>(SLC7A11)</t> and SOD-3. (B-D) Statistical analysis of the western blots. Neither Reox nor CH223191 affects Nrf2 activity, or the expression of xCT and SOD-3. Data are presented as the mean ± SEM of six independent experiments. AhR, arylhydrocarbon receptor; Nrf2, nuclear factor erythroid 2-related factor 2; xCT, cystine-glutamate <t>antiporter;</t> SOD-3, superoxide dismutase; RPTEC, renal proximal tubular epithelial cell; ctrl, control; Reox, reoxygenation.
Cystine Glutamate Antiporter, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine (L-BPA). Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- BPA uptake by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.

Journal: bioRxiv

Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages

doi: 10.1101/2025.02.20.639245

Figure Lengend Snippet: (A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine (L-BPA). Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- BPA uptake by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.

Article Snippet: BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.

Techniques: Membrane, Infection, Control, Fluorescence, Comparison

AhR activation status does not affect Nrf2 activation or transcriptional activity. RPTECs were cultured under ctrl conditions or subjected to Reox with or without the AhR inhibitor CH223191. (A) Representative western blots of Nrf2 levels (corresponding to its activation status) and the expression of the Nrf2 transcriptional targets xCT (SLC7A11) and SOD-3. (B-D) Statistical analysis of the western blots. Neither Reox nor CH223191 affects Nrf2 activity, or the expression of xCT and SOD-3. Data are presented as the mean ± SEM of six independent experiments. AhR, arylhydrocarbon receptor; Nrf2, nuclear factor erythroid 2-related factor 2; xCT, cystine-glutamate antiporter; SOD-3, superoxide dismutase; RPTEC, renal proximal tubular epithelial cell; ctrl, control; Reox, reoxygenation.

Journal: Molecular Medicine Reports

Article Title: Reoxygenation induces reactive oxygen species production and ferroptosis in renal tubular epithelial cells by activating aryl hydrocarbon receptor

doi: 10.3892/mmr.2020.11679

Figure Lengend Snippet: AhR activation status does not affect Nrf2 activation or transcriptional activity. RPTECs were cultured under ctrl conditions or subjected to Reox with or without the AhR inhibitor CH223191. (A) Representative western blots of Nrf2 levels (corresponding to its activation status) and the expression of the Nrf2 transcriptional targets xCT (SLC7A11) and SOD-3. (B-D) Statistical analysis of the western blots. Neither Reox nor CH223191 affects Nrf2 activity, or the expression of xCT and SOD-3. Data are presented as the mean ± SEM of six independent experiments. AhR, arylhydrocarbon receptor; Nrf2, nuclear factor erythroid 2-related factor 2; xCT, cystine-glutamate antiporter; SOD-3, superoxide dismutase; RPTEC, renal proximal tubular epithelial cell; ctrl, control; Reox, reoxygenation.

Article Snippet: Primary antibodies were specific for AhR (1:200; cat. no. sc-133088; Santa Cruz Biotechnology, Inc.), cytochrome P450 family 1 subfamily A member 1 (CYP1A1; 1:500; cat. no. sc-25304; Santa Cruz Biotechnology, Inc.), Nrf2 (1:1,000; cat. no. TA343586; OriGene Technologies, Inc.), superoxide dismutase 3 (SOD-3; 1:100; cat. no. sc-271170; Santa Cruz Biotechnology, Inc.), cystine-glutamate antiporter (xCT, also known as SLC7A11; 1:1,000; cat. no. ANT-111; Alomone Labs), HIF-1α (1:500; cat. no. sc-10790; Santa Cruz Biotechnology, Inc.), LDH-A (1:1,000; cat. no. 2012; Cell Signaling Technology, Inc.), activated cleaved caspase-3 (CC3; 1:1,000; cat. no. ab13847; Abcam) and β-actin (1:2,500; cat. no. 4967; Cell Signaling Technology, Inc.).

Techniques: Activation Assay, Activity Assay, Cell Culture, Western Blot, Expressing, Control